Titlebook: Detection of Cell Death Mechanisms; Methods and Protocol Ayesha B. Alvero,Gil G. Mor Book 2021 Springer Science+Business Media, LLC, part o

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In Vitro Identification and Isolation of Human Neutrophil Extracellular Traps,perties and their role in pathophysiological processes is a rapidly evolving and expanding field. Here, we describe a combination of methods to achieve a successful in vitro NET visualization, semiquantification, and isolation.

SHRIK

Induction and Detection of Necroptotic Cell Death in Mammalian Cell Culture,ive controls, detection of necroptosis mediator proteins via Western Blot analysis, and interpretation of results. This protocol allows reliable and specific detection of necroptosis in cell culture or tissue samples, and it provides a well-established model suitable for detailed studies of necroptosis molecular mechanisms in vitro.

Asparagus

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Blutung in der Nachgeburtsperiode,orescence intensity correlated with the degree of secondary necrosis. Altogether, the measures provide exquisite kinetic resolution of dose- and agent-dependent apoptotic responses, from early through late phases. At exposure termination, other compatible reagents can be applied to measure additional orthogonal correlates of cell health.

CURT

VERT

A Real-Time, Bioluminescent Apoptosis Assay,orescence intensity correlated with the degree of secondary necrosis. Altogether, the measures provide exquisite kinetic resolution of dose- and agent-dependent apoptotic responses, from early through late phases. At exposure termination, other compatible reagents can be applied to measure additional orthogonal correlates of cell health.

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