Titlebook: Detection of Cell Death Mechanisms; Methods and Protocol Ayesha B. Alvero,Gil G. Mor Book 2021 Springer Science+Business Media, LLC, part o

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Visualization of Necroptotic Cell Death through Transmission Electron Microscopy,rent chapter provides detailed guidelines for imaging morphological changes during programmed cell necrosis using TEM as a single-step methodology. In this protocol, a novel aldehyde dehydrogenase inhibitor is used to induce cell programmed necrosis in ovarian cancer cell lines (A2780 and SKOV3). Th

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A Luminescence Assay to Quantify Cell Viability in Real Time, from hours to several days. Here, we describe a sensitive, nonlytic live-cell assay that allows continuous or ‘real-time’ monitoring of cell viability, growth, and cytotoxicity over an extended period of time. We illustrate the use of the assay for small drug molecule and antibody-dependent cytotox

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1064-3745 ation advice from the experts.This volume provides detailed protocols for the performance, analysis, and troubleshooting of?.in vitro.?and?.in vivo.?experiments related to programmed cell death. Chapters compile?conventional techniques such as?western blot and qPCR and state-of-the art transmission

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Book 2021med cell death. Chapters compile?conventional techniques such as?western blot and qPCR and state-of-the art transmission electron microscopy and real-time multiplexed imaging assays. ?Written in the highly successful?.Methods in Molecular Biology.?series format, chapters include introductions to the

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Book 2021ir respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and accessible,?.Detection of Cell Death Mechanisms: Methods and Protocols?.aims to ensure seamless execution of protocols on specific cell death type..