Titlebook: DNA Repair Protocols; Lotte Bjergb?k Book 2012Latest edition Springer Science+Business Media New York 2012 Arabidopsis cell extracts.DNA r

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Patrick Orth MD,Henning Madry MD homologous recombination and translesion synthesis activities using activation-induced deaminase (AID)-induced diversification of the immunoglobulin locus. In this chapter, we would describe a detailed protocol for gene disruption experiments in DT40 cells.

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Zhao Lilian,Li Yanjin,Fu Chuyingd organ cultures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.

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Quantification of DNA Photoproducts in Mammalian Cell DNA Using Radioimmunoassayd organ cultures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.

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In Vitro DNA Mismatch Repair in Human Cellss required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and the repair assay. In this chapter, we describe the detailed methods for this functional in vitro assay.

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Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-Mediated PCRapping of DNA lesions in mammalian cells is the ligation-mediated polymerase chain reaction (LM-PCR) technique. We provide an update on technical details of LM-PCR. LM-PCR can be used, for example, for mapping of ultraviolet (UV) light-induced DNA photoproducts such as cyclobutane pyrimidine dimers.