Titlebook: DNA Nanotechnology; Methods and Protocol Giampaolo Zuccheri Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation oflar detection or separation. We here provide a protocol to perform capture-SELEX for DNA aptamers binding soluble small molecules, which includes a straightforward functional validation by SPR. The SELEX strategy here described is adaptable to any water-soluble molecular target and might foster the

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A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems,ethod has emerged as a powerful computational tool for atomic-resolution characterization of the in situ structure and physical properties of DNA origami objects. This chapter provides step-by-step instructions for building atomic-scale models of DNA origami systems, using the MD method to simulate

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Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform,th single-molecule control. We combine the ability of DNA nanostructures as programmable platforms, with a one-step Focused Ion Beam nanopatterning, to demonstrate the controlled immobilization of DNA origami functionalized with individual quantum dots (QDs) at predesigned positions on glass coversl

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DNA Origami Structures Interfaced to Inorganic Nanodevices,DNA origami and incubate them onto arrays of anchoring gold nanodots either in a static solution or in a microfluidic system. We give details on the design and lithographic methods employed to pattern usable arrays of gold nanoanchors on naturally oxidized silicon wafer chips. Scanning electron and

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Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology,at the nanoscale. Here we show how it is possible to control the plasmonic resonance of complex architectures of gold nanoparticles using the peculiar properties of DNA Watson and Crick pairing rules. In this chapter, we will first introduce all the steps required to generate, purify, and characteri