Titlebook: DNA Microarrays for Biomedical Research; Methods and Protocol Martin‘Dufva Book 20091st edition Humana Press 2009 Allele.DNA.Gene expressio

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Immobilization Chemistries,a decisive role..This article attempts to review the main concepts and ideas of the multiple variants which exist in terms of the immobilization chemistries used in nucleic acid microarray technology. Starting from the attachment of unmodified nucleic acids to modified glass slides by adsorption, fu

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RNA Preparation and Characterization for Gene Expression Studies,ology as little as a single cell is required as starting material for gene expression experiments. The mRNA from a single cell may be linearly amplified to an excess of 10.-fold. Reverse transcription and fluorescent labeling of the amplified RNA yields a stable target for subsequent hybridization t

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Gene Expression Analysis Using Agilent DNA Microarrays,dardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridiz

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Target Preparation for Genotyping Specific Genes or Gene Segments,lished for production of single stranded DNA and in vitro transcribed RNA. Here we describe three robust methods for target amplification from purified genomic DNA or pre-amplified DNA. The protocols include incorporation of biotin labels in the target molecules and allow for biotin/streptavidin che

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Genotyping of Mutations in the Beta-Globin Gene Using Allele Specific Hybridization,oarray-based assay using allele-specific oligonucleotide (ASO) probes for genotyping mutations in the beta-globin gene. The assay makes use of agarose film coated glass slides as substrate, unmodified melting temperature matched ASO probes, target amplification and labeling using T7 in vitro transcr

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