Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin

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Book 2020synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions

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Orientierung an Gebrauchswertenthe structure of the final construct to identify all basic parts and vectors required for the construction strategy. The assembly strategy is then defined following a set of standard rules. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.

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Assembly of Multigene Constructs Using the Modular Cloning System MoClothe structure of the final construct to identify all basic parts and vectors required for the construction strategy. The assembly strategy is then defined following a set of standard rules. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.

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Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plantsdesign and construct new Phytobricks as well as how to assemble them in both manual and nanoscale automated one-step reactions. Finally, we describe a high-throughput method for sequence verification of assembled plasmids.

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Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with s. Here we examine available DNA assembly methods and describe through example, the construction of a complex but not atypical combinatorial and hierarchical library using protocols that are generated automatically with the assistance of modern synthetic biology software.

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