Titlebook: cDNA Library Protocols; Ian G. Cowell,Caroline A. Austin Book 1997 Springer Science+Business Media New York 1997

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書目名稱cDNA Library Protocols被引頻次




書目名稱cDNA Library Protocols被引頻次學(xué)科排名




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親密

Applications in Statistical Computingn the λ vector (.). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.

debble

華而不實(shí)

,Clone Excision Methods for the Lambda ZAP?-Based Vectors,n the λ vector (.). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.

饑荒

Review of the Development of Energy Finance cells in a tissue are expressing genes of interest or the tissue is in limited supply. A cDNA library from the targeted cells is preferable to a library constructed from the entire tissue containing these cells, because in the latter case, genes expressed in the targeted cells may be too rare to be

OCTO

Sibel Soylu,?lkay ?endeniz-Yüncü,U?ur Soyta?r regionally, temporally, or environmentally specific), a large proportion of all transcripts (approx 40–45%) represent low-abundance mRNAs, present at 1–20 molecules/cell (.). In a given cell type, “l(fā)ow-abundance” mRNAs are likely to represent >95% of the different mRNAs expressed. The lower the ab

OCTO

Review of the Development of Energy Financeof RNA can be made from several grams of tissue or as little as 50 mg. However, large samples are generally more representative of the genes expressed in a population of plants in response to environmental cues or at a defined stage of development. Therefore, large-scale extraction of RNA is the met

slow-wave-sleep

大廳

開始沒有

Wolfgang Gaul,Christoph Winkler The approach described here to clone the missing sequence (cDNA ends) employs polymerase chain reaction (PCR). Since the initial reports of rapid amplification of cDNA ends (RACE) (.) or related techniques (.,.), many labs have developed significant improvements on the basic approach (.–.). The mos