Titlebook: Cytoskeleton Methods and Protocols; Ray H. Gavin Book 2016Latest edition Springer Science+Business Media New York 2016 Cell and Organelle

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https://doi.org/10.1007/978-3-662-11140-6e developed an in vitro system that combines micromanipulation of force by magnetic tweezers with simultaneous live cell fluorescence microscopy. We applied pulling forces to magnetic beads coated with the . Type IV pili in the same order of magnitude than the forces generated by live bacteria. We s

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https://doi.org/10.1007/978-3-662-11140-6oteins. Traditional approaches make use of protein overexpression or siRNA. However to study or modulate resident endogenous proteins, complementary methods are required. Since the discovery of nanobodies in 1993, they have proven to represent interesting tools in a variety of applications due to th

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An?sthesie bei seltenen Erkrankungen on morphogenesis, motility, organelle movement, or when apoptosis has to be induced. These substances can be divided into two major subclasses: F-actin-stabilizing and -polymerizing substances like jasplakinolide and chondramides and F-actin-severing compounds like chytochalasins and latrunculins.

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An?sthesie bei seltenen Erkrankungen analysis of dynamic cell shape changes that occur through extension and retraction of force generating structures such as pseudopodia and lamellipodia. Quantitative analysis of these structures is an underutilized tool in the field of cell migration. Our intent, therefore, is to present methods tha

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