Titlebook: Cytokine Bioassays; Methods and Protocol Ivana Vancurova Book 2014 Springer Science+Business Media New York 2014 Cytokines.RT-PCR.analysis

Narrative

https://doi.org/10.1007/978-94-009-7630-6se, cell proliferation, migration, and survival. IL-8 expression is increased not only in inflammatory disorders, but also in many types of cancer, including prostate cancer. IL-8 acts as a ligand for the C-X-C chemokine receptor 2 (CXCR2) protein present on the cell plasma membrane. Binding of the

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interference

https://doi.org/10.1007/978-1-4939-0928-5Cytokines; RT-PCR; analysis of intracellular cytokines by flow cytometry; biologically active cytokines

guardianship

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A Useful Guide for Analysis of Immune Markers by Fluorochrome (Luminex) Techniquelear cells (PBMC)..This chapter describes the Luminex technique for detection of multiple cytokines by magnetic bead sandwich immunoassay, with a special focus on some important pre-analytical factors, such as cell separation, cryopreservation, and PBMC thawing that may affect the detection outcome