Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.Genomics.H

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Channel Surfers: ,’s Postnational Fansf treatment. We provide here detailed protocols and tips for collecting and preserving tissues and cells, and preparing total RNA. We also outline novel strategies for experimental design and data analysis, and describe some powerful gene and pathway discovery tools.

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Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibathogenesis of CF airway disease. Hence, assessment of Ca.. signaling has become an important area of CF research. This chapter will focus on measurements of cytoplasmic and mitochondrial Ca. signals resulting from GPCR activation in polarized primary cultures of normal and CF human bronchial epithelia (HBE).

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