Titlebook: Cystic Fibrosis; Diagnosis and Protoc Margarida D. Amaral,Karl Kunzelmann Book 2011 Springer Science+Business Media, LLC 2011 CF.Genomics.H

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Methods for ASL Measurements and Mucus Transport Rates in Cell Culturesuce and are bathed by a layer of airway surface liquid (ASL), which plays a critical role in lung defense by helping to maintain the sterility of the lung. This layer is composed of two phases: the mucus layer which functions to trap particulates, bacteria, and viruses, and the underlying periciliar

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Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibfor G protein-coupled receptors (GPCR). Intracellular calcium (Ca..) mobilization in airway epithelia in response to extracellular stimuli regulates key airway innate defense functions, e.g., Ca.-activated Cl. secretion, ciliary beating, mucin secretion, and inflammatory responses. Because Ca.. mobi

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Microarray mRNA Expression Profiling to Study Cystic Fibrosisaffected cell types. Microarray expression profiling provides a platform to study global gene expression in detail. This approach may provide the necessary information to segregate phenotypic characteristics of CF, differentiate between genetic or environmental factors, and assess the advent and pro

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