Titlebook: Current Laboratory Methods in Neuroscience Research; Huangui Xiong,Howard E. Gendelman Book 2014 Springer Science+Business Media New York

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Oocytes Isolation and Microinjectionnels and receptors. The ovarian tissue can be extracted either by a nonfatal surgical removal of parts of the ovary on an anesthetized frog several times during its lifetime or by removal of the ovary from the frog decapitated after deep anesthesia. Then, the . oocytes can be isolated and defollicul

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Polymerase Chain Reaction (PCR) and Real-Time PCRmolecular cloning, diagnosis of infectious and hereditary diseases, and forensic sciences. Improvements of the PCR technique have resulted in its expansion to include variants such as reverse-transcription PCR, real-time PCR, and real-time reverse-transcription PCR. This chapter provides an overview