Titlebook: Crystallographic Methods and Protocols; Christopher Jones,Barbara Mulloy,Mark R. Sanderson Book 1996 Humana Press 1996

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The Foreign Policy Election That Wasn’ts (.–.) have been obtained by this technique. Electron crystallography seems particularly appropriate for membrane proteins that are prone to form 2D crystals, and it is hoped that many more structures will be determined in this way.

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Modern Methods for Rapid X-Ray Diffraction Data Collection from Crystals of Macromolecules,in a glass or quartz capillary tube is usually held using plasticene and then aligned in the X-ray beam. This chapter will outline the principles of the most commonly used laboratory equipment, and give the basic steps involved in data collection and processing.

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Density Modification in X-Ray Crystallography,bio-polymer chain, and known noncrystallographic symmetry of the density distribution. To impose the physical constraints on an experimental map, an iterative algorithm has been proposed (.,.). It alternates real and reciprocal space operations, and merges gradually the physical constraints with the initial amplitudes and phases.

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There may be more than one subunit in the crystallographic asymmetric unit. The similarity may be only partial so that a fragment is used. Examples include related structures, site-directed mutants, structures with ligands, and Fab-antigen complexes.