Titlebook: Cryptosporidium; Methods and Protocol Jan R. Mead,Michael J. Arrowood Book 2020 Springer Science+Business Media, LLC, part of Springer Natu

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A Practical Guide to Study Design,nd ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to . isolation or nucleic acid extraction methods for the detection of . oocysts or . DNA.

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Enterprise Business Integration in 2010A.D.ut screening (HTS) of drugs against . in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of . in vitro.

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https://doi.org/10.1007/978-1-4939-9748-0Protozoan; Intestinal tract infection; Anti-cryptosporidial drugs; Genotyping; Oocysts; CRISPR/Cas9; Host-

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Fragrance Materials in Wastewater Treatment,from stool and intestine of infected mice to facilitate oocyst quantification. Moreover, we present protocols using flow cytometry, quantitative polymerase chain reaction, and histopathology to accurately quantify parasite burden in stool or intestine samples.

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Vladimir Tosic,Kruti Patel,Bernard Pagurekssay readout. Here we describe an established 384- or 1536-well format high-content imaging (HCI) assay of .-infected HCT-8 human ileocecal adenocarcinoma cells. This HCS assay is a powerful tool to assess large numbers of compounds to power drug discovery, as well as to phenotypically characterize known .-active compounds.

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Mouse Models for Use in , Infection Studies and Quantification of Parasite Burden Using Flow Cytomefrom stool and intestine of infected mice to facilitate oocyst quantification. Moreover, we present protocols using flow cytometry, quantitative polymerase chain reaction, and histopathology to accurately quantify parasite burden in stool or intestine samples.

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