Titlebook: Cryptococcus neoformans; Methods and Protocol Erin E. McClelland Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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Chunming Yang,Xinghao Song,Hui Zhang,Bo Liesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating the average number of yeast per host cell and a fluorescence microscopy-based method that may be used to measure changes in fungal burden in individual living macrophages in real time.

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Hermeneutics, Social Theory and Education three commonly used strategies to establish cryptococcosis in mice: intranasal, intratracheal, and intravenous inoculations. Also, we discuss the methodology for delivering drugs to mice via intraperitoneal injection.

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Chunming Yang,Xinghao Song,Hui Zhang,Bo Liibe sample preparation, followed by purification, processing on the mass spectrometer, and a robust bioinformatics analysis. The information gleaned from proteomic profiling of fungal infections supports the detection of novel biomarkers for diagnostic and prognostic purposes.

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