Titlebook: Cryotechniques in Biological Electron Microscopy; Rudolf Alexander Steinbrecht,Karl Zierold Book 1987 Springer-Verlag Berlin Heidelberg 19

Geyser

Pruritus

High Resolution Metal Replication of Freeze-Dried Specimensspecimens (Nermut and Frank 1971; Kistler et al. 1977; Gross et al. 1985). Very thin specimens such as isolated membranes or isolated macromolecules can be directly adsorbed on carbon-coated grids. After rapid freezing, the grids are transferred into suitable high-vacuum equipment for freeze-drying

門閂

過分自信

Cryoultramicrotomy for Autoradiography and Enzyme Cytochemistrycytochemistry, the classical methods in histochemistry and cytochemistry. It is an unpleasant task to summarize the present situation and to arrive at the conclusion that these promises are only partly fulfilled. For the autoradiography of diffusible substances, there seem to be only practical limit

expository

Scanning Electron Microscopy and X-Ray Microanalysis of Frozen-Hydrated Bulk Samplesntation of the living state that is currently possible to obtain in the electron microscope. An exception is the “environmental microscope” (Danilatos and Postle 1982), but this has considerably more limitations in its use than the “l(fā)ow temperature microscope”. The methods and techniques of low temp

極肥胖

Cryofixation of Dynamic Processes in Cells and Organellesponents (Plattner and Bachmann 1982). This intention of fixation is, of course, not restricted to applications in electron microscopy. Attempts to literally “freeze” the living structure by cryofixation can be traced back to the last century, and were extended to electron microscopy in its very earl

Neutral-Spine

Cryofixation of Diffusible Elements in Cells and Tissues for Electron Probe Microanalysisprinciple, the irradiating electron beam (probe) is focussed on the area of interest in the specimen, while the secondary radiation (e.g. X-rays) generated by the interaction with the specimen is processed through a spectrometer to provide information on the kind and amount of the elements present.

capsaicin

Wenxing Zhong,Dehong Xu,Ron Shu Yuen Huihieved with procedures that allow for averaging from identical subunits. At the other level, we consider rearrangements and small aggregations of macromolecules due to the specimen preparation. This is the resolution of 10–20 nm achieved normally with the current methods of preparation of biological material.

通便

wall-stress