Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harri?tte Oldenhof Book 2015Latest edition Springer Science+Business Media

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Laser Scanning Microscopy in Cryobiologyuring freezing and thawing of a sample using a temperature-controlled sample holder. In this chapter, various advanced cryoimaging methods are described. Special emphasis is given on the different imaging modalities that can be used to study the various aspects of cryopreservation.

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Low-Temperature Electron Microscopy: Techniques and Protocolshich are commonly encountered in more conventional room temperature electron microscopy techniques which rely heavily on chemical fixation and heavy metal staining. Often, cryo-electron microscopy allows direct observation of specimens, which have not been stained or chemically fixed.

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Calorimetric Analysis of Cryopreservation and Freeze-Drying Formulations the optimization of protective formulations and process protocols. This chapter gives an introduction to beginners who are less familiar with this technique. It covers the instrument and its basic principles, followed by a discussion of the methods as well as examples of specific applications.

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Efficient Cryopreservation of Human Pluripotent Stem Cells by Surface-Based Vitrificationon a procedure for human embryonic stem cells developed by Beier et al. (Cryobiology 63:175–185, 2011). This simple and highly efficient cryopreservation method allows cryopreservation of large numbers of ready-to-use adherent cells that maintain pluripotency.

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Chenshu Wu,Zheng Yang,Yunhao Liucribed. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.

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Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cellscribed. Special emphasis is given on studying membrane phase behavior and protein denaturation in cells or tissues. In addition, methods are presented to determine the water-to-ice phase change during freezing, dehydration kinetics, and the glass transition temperature of amorphous systems.

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