Titlebook: Cryopreservation and Freeze-Drying Protocols; John G. Day,Glyn N. Stacey Book 2007Latest edition Humana Press 2007 biological materials.bi

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Cryopreservation of Microalgae and Cyanobacteria,tic algae can also be cryopreserved, but typically with lower post-thaw viability levels. However, to date, most dinoflagellates, cryptophytes, synurophytes, and raphidophytes cannot be successfully cryopreserved. Marine diatoms can be cryopreserved, and often have high viability, although freshwate

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Cryopreservation of Plant Cell Suspensions,tion of higher plant germplasm. Suspension cultures are also important in biotechnology, particularly in transformation studies and for the production of specific metabolites, and, here, there is also a pressing need for genetically stable, long-term storage of cell lines..Cryopreservation of suspen

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Cryopreservation of Avian Spermatozoa,nto liquid nitrogen with dimethylacetamide as cryoprotectant. The method originates from the group at the Research Institute of Farm Animal Breeding and Genetics at St. Petersburg-Pushkin and is described here for chicken spermatozoa, but has also been adapted successfully for other species, such as

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Cryopreservation of Animal and Human Cell Lines,intain the increasing numbers of cell lines, as they started to emerge from research laboratories from the mid-1900s, necessitated a radical approach to the problem. The realization that they could survive cryopreservation, and that a slow rate of cooling is essential for this survival, led to the e

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Cryopreservation of Hematopoietic Stem/Progenitor Cells for Therapeutic Use,cal malignancies. At least 70% of these are autologous transplants, the remaining 30% being allogeneic, which are sourced from related (70% of the allogeneic) or unrelated donors. Peripheral blood mobilized with granulocyte colony stimulating factor is the major source of stem cells for transplantat

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Cryopreservation of Human Embryonic Stem Cell Lines,/rapid warming. The vitrification method described here is designed for hESCs that grow as discrete colonies on a feeder cell monolayer, and are subcultured by manual subdivision of the colonies into multicellular clumps. hESCs that are subcultured by enzymatic dissociation can more conveniently be

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