Titlebook: Coronaviruses; Methods and Protocol Helena Jane Maier,Erica Bickerton,Paul Britton Book 2015 Springer Science+Business Media New York 2015

FRET

Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomeserious restrictions for the development of CoV infectious clones using reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these problems, several approaches have been established in the last 13 years. Here we describe the engineering of CoV full-length

CULP

Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Rral and host determinants involved in the first step in the infection of a host cell by a virus. Applying recombinantly expressed spike proteins of infectious bronchitis virus onto formalin-fixed tissues allows us to profile the binding characteristics of these viral attachment proteins to tissues o

噴油井

桶去微染

夜晚

Isolation and Propagation of Coronaviruses in Embryonated Eggsbranes and associated allantoic fluids. TCoV replicates only in epithelium lining the embryo intestines and bursa of Fabricius, thus amniotic inoculation is required for isolation and propagation of this virus. Embryonated eggs also provide a potential host system for detection and characterization

BRIEF

medium

Glutinous

得罪

Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomicsent responsible for the fulminating disease of guinea fowl, we identified a novel avian gammacoronavirus associated with the disease using metagenomics. Next-generation sequencing is an unbiased approach that allows the sequencing of virtually all the genetic material present in a given sample.

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