Titlebook: Confocal Microscopy; Methods and Protocol Joseph Brzostowski,Haewon Sohn Book 2021 This is a U.S. government work and not under copyright p

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1064-3745 ding known pitfalls..?..Authoritative and cutting-edge,?.Confocal Microscopy: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-0716-1404-4978-1-0716-1402-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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tional confocal function, the Airyscan unit comes as an add-on to the conventional Zeiss laser scanning confocal microscope. This protocol is for the first generation Airyscan Zeiss 800 series microscope.

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e use of Mitotracker dyes) while efficiently excluding nonviable cells. Our novel imaging strategy offers a powerful tool to study changes in mitochondrial morphology and complements any research focusing on lymphocyte metabolism.

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these cells. Lastly, we describe two types of quantitative analysis: vesicle distribution/clustering toward?the microtubule organizing center (MTOC), and colocalization analysis with endolysosomal markers.

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.infected RBCs. The data shared with this protocol reveals that there is a significant overall increase in autofluorescence lifetime in infected erythrocytes compared to the healthy uninfected ones. We include a metabolic experiment that confirms that the signals obtained from this imaging technique

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Choosing Fluorescent Probes and Labeling Systems,s when designing an experiment is the selection of an appropriate fluorescent probe. Equally important is deciding how the probe will be attached to the protein of interest. The advantages and disadvantages of different fluorescent probe types and their respective labeling methods are discussed to p

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Microfabricated Devices for Confocal Microscopy on Biological Samples,d and multiple commercial devices now available. This chapter is intended to provide an overview of the available options for devices compatible with confocal microscopy, including an overview of fabrication techniques and some examples of device use. Although there are times when off-the-shelf devi