Titlebook: Computer-Aided Antibody Design; Kouhei Tsumoto,Daisuke Kuroda Book 2023 Springer Science+Business Media, LLC, part of Springer Nature 2023

AGATE

不妥協(xié)

疏遠(yuǎn)天際

Assessing and Engineering Antibody Stability Using Experimental and Computational Methods molecules with the desired conformational and colloidal stability. This chapter introduces our methods to computationally characterize an antibody Fab fragment, propose stabilizing variants, and then experimentally verify these predictions.

A簡潔的

scrutiny

unstable-angina

Ambiguous

挑剔為人

Thermal Stability Estimation of Single Domain Antibodies Using Molecular Dynamics Simulations to improve the stability and have later been validated by experiments. Our protocol extends beyond the application on sdAbs, as it is also suitable for other proteins and to determine the interfacial stability between protein and ligand.

adumbrate

空中

Structure-Based Optimization of Antibody-Based Biotherapeutics for Improved Developability: A Practif candidates arising from target affinity screening and to optimize lead candidates for developability. Methods for modeling antibody structures from sequence and detecting post-translational modifications and chemical degradation liabilities are also discussed.