Titlebook: Computational Design of Ligand Binding Proteins; Barry L. Stoddard Book 2016 Springer Science+Business Media New York 2016 ligand-binding

agitate

Struktur der erzeugenden Distributionen, computational design, iterative site saturation mutagenesis, and yeast two-hybrid system (Y2H)-based phenotypic screening to engineer novel and orthogonal interactions between synthetic ligands and human estrogen receptor α (hERα) for the development of novel gene switches.

Conscientious

Rosetta and the Design of Ligand Binding Sites, redesign an existing protein pocket to bind a target ligand. Starting with a protein structure and the structure of the ligand, Rosetta can optimize both the placement of the ligand in the pocket and the identity and conformation of the surrounding sidechains, yielding proteins that bind the target compound.