Titlebook: Combinatorial Peptide Library Protocols; Shmuel Cabilly Book 1998 Humana Press 1998

間接

Adenocarcinoma

GIBE

Construction and Use of a 20-mer Phage Display Epitope Library,xpression systems have been developed in order to construct such libraries containing tens to hundreds of mlllions of random peptlde sequences that can be screened for their abihty to bind particular antibodies (.). In essence these phage-expressed peptides mimic the determinants of the antigen that are recognized by the antibodies.

光亮

avulsion

Kusum Lata,Priyanka Kumar,Arpita Banerjeenique for screening combinatorial libraries employing recombinant receptors expressed in the plasma membrane of transfected melanophors (.,. and Chapters 13,14). Their technique is based on the biological activity of the expressed receptors and it enables the selection of library members with agonistic or antagonistic activities.

頂點(diǎn)

Peralkylation,CLs used as starting materials. The screening of a SCL composed of 50 million peptidomimetics (.) has yielded individual active compounds that had no homology to those found in the starting SCLs. We describe here improved methods (.) developed for the transformation of such libraries.

Asymptomatic

Exaggerate

Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports,is of thousands of peptrdes or peptide mixtures bound to continuous cellulose membrane supports. Presently up to 8000 different spots (peptides or peptide mixtures) can be automatically synthesized on a 20 *x 30-cm cellulose membrane and screened for ligand binding within l–2 wk.

Fortuitous

Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIIIing both recombinant and wild-type proteins. The high copy number of recombinant pVIII molecules per phage particle provides a highly sensitive system, and linear and constrained peptide libraries in pVIII have been successfully used for the selection of specific ligands for several different monoclonal and polyclonal antibodies (.).

Omnipotent

1064-3745 ving organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutiona