Titlebook: Clinical Applications of PCR; Y. M. Dennis Lo,Rossa W. K. Chiu,K. C. Allen Chan Book 2006 Humana Press 2006 DNA.Laboratory.Mutation.PCR.ge

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1064-3745 ids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.978-1-61737-511-8978-1-59745-074-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatographyanalytical separations of DNA based on temperature: analysis is performed at a temperature sufficient to partially denature DNA heteroduplexes. The technology detects single-base changes as efficiently as short deletions and insertions. The chance that a mutation cannot be detected is 0.5%.

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Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies,possible only a few years ago. Examples are the analysis of gene expression or gene duplications/losses, where twofold differences in nucleic acid concentration have routinely been determined with almost 100% accuracy. As our primary interest is in prenatal diagnosis, we have investigated whether re