Titlebook: Clathrin-Mediated Endocytosis; Methods and Protocol Laura E.‘Swan Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2

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BIPED

Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System,iously described an assay of reconstituting ccp assembly on sheets of basal plasma membranes. Here, we describe a workflow to adapt this system for monitoring the assembly of ccps over time using total internal reflection fluorescence (TIRF) microscopy.

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Stimulated Emission Depletion (STED) Imaging of Clathrin-Mediated Endocytosis in Living Cells,ular processes with unprecedented details. Here we describe how to image endocytic events at the plasma membrane of living cells using commercial (Leica, Abberior Instruments) or custom built STED microscopes.

NEX

Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution,ty information, imaging experiments can be rendered quantitative, yielding insights into the stoichiometry and kinetics of the components of a molecular assembly. Here, we describe the experimental and analytical steps needed to study the assembly of clathrin-coated vesicles with single-molecule res

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Euthyroid

Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture,ne bound are intensely fluorescent, combined with the flexibility of different emission wavelengths make these dyes an excellent choice for investigating clathrin-mediated endocytosis, among other membrane trafficking and recycling pathways.

CANON

大廳

978-1-4939-9374-1Springer Science+Business Media, LLC, part of Springer Nature 2018

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