Titlebook: Clathrin-Mediated Endocytosis; Methods and Protocol Laura E.‘Swan Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2

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Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifundamental to study the biochemistry and the physiology of the nervous system. This chapter describes the isolation and purification of intact synaptosomes from rodent brain tissue that can be used to further characterize synaptic structure and function and to examine the molecular mechanisms of neurotransmission.

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Cryo-Electron Tomography of the Mammalian Synapse, unstained, fully hydrated cellular structures at molecular resolution. The protocol focuses on purified synaptic terminals (“synaptosomes”), currently the most suitable preparation to analyze mammalian synaptic architecture by cryo-ET.

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,Imaging “Hot-Wired” Clathrin-Mediated Endocytosis,d in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.

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