Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2023Latest edition The Editor(s) (if applicable) and The

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Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Liunction as parts of bispecific IgG-like symmetrical antibodies when they replace their wild-type Fc. Their homodimeric structure typically leads to bivalent antigen binding. In particular, biological situations monovalent engagement, however, would be preferred, either for avoiding agonistic effects

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Single-Cell B-Cell Sequencing to Generate Natively Paired scFab Yeast Surface Display Libraries,onoclonal antibodies (mAbs). Using scRNA-seq to determine natively paired B-cell receptor (BCR) sequences of immunized mice as a starting point for design, this method outlines a simplified workflow to express single-chain antibody fragments (scFabs) on the surface of yeast for high-throughput chara

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Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening,tion with other molecules. The inability to reach the binding pocket can impose a substantial or even insurmountable barrier for the de novo identification or optimization of small-molecule ligands. Herein, we describe a protocol for the engineering of a target protein and a yeast display FACS sorti

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