Titlebook: Cis/Transgene Optimization; Systematic Discovery Saeid Kadkhodaei,Farahnaz Sadat Golestan Hashemi,A Book 2018 The Author(s) 2018 Cis/transg

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2193-4746 onal biology applications.Offers step-by-step procedures for.This book is a practical review which focuses on computational analysis and on .in silico. approaches towards the systematic discovery of various key functional gene expression elements in microalgae as a model. So far, in this regard very

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https://doi.org/10.1007/978-2-287-72827-3nd stability. The outlines can be efficiently deployed as a practical model for systematic discovery of the key expression elements and optimization of the cis/transgenes in other micro/organisms in which there is limited information about these factors.

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Outcomes Assessment,uence considering the essential motifs and underlain rules. As the consensus TIS, we realized the specific context in the microalgae which associates with locating the ribosome at the correct reading frame. This study also reviews the application of various . tools and databases to minimize the time

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Conceptual Models,y set out with the aim of systematic discovery of the key upstream factors for genetic manipulation of microalgae as a case study. The review indicates how the MARs flanking the expression cassette along with the optimized expression elements could potentially improve the transformation efficiency a