Titlebook: Circular RNAs; Methods and Protocol Christoph Dieterich,Argyris Papantonis Book 2018 Springer Science+Business Media, LLC, part of Springer

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書(shū)目名稱Circular RNAs影響因子(影響力)




書(shū)目名稱Circular RNAs影響因子(影響力)學(xué)科排名




書(shū)目名稱Circular RNAs網(wǎng)絡(luò)公開(kāi)度




書(shū)目名稱Circular RNAs網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書(shū)目名稱Circular RNAs被引頻次




書(shū)目名稱Circular RNAs被引頻次學(xué)科排名




書(shū)目名稱Circular RNAs年度引用




書(shū)目名稱Circular RNAs年度引用學(xué)科排名




書(shū)目名稱Circular RNAs讀者反饋




書(shū)目名稱Circular RNAs讀者反饋學(xué)科排名

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978-1-4939-8521-0Springer Science+Business Media, LLC, part of Springer Nature 2018

Alopecia-Areata

Circular RNAs978-1-4939-7562-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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斜

https://doi.org/10.1007/978-3-642-54508-5t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

斜

Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR,plice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.

滲透

Nonradioactive Northern Blot of circRNAs,y to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (.P) labeled, which is not environment-friendly and sometimes inconvenient to use. Here, we describe a nonradioactive northern blot protocol with digoxigenin-labeled probe to detect circRNA.

mortgage

Preparation of Circular RNA In Vitro,t the 5′ end is first prepared by a chemical or enzymatic method, then circularized using ligase. The function of the prepared circular RNA molecule, such as an ability to induce translation, can then be investigated.

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Christoph Dieterich,Argyris PapantonisIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts