Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu

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Base-Editor-Mediated circRNA Knockout by Targeting Predominantly Back-Splice Sitesg sequences to their cognate linear RNAs from the same gene loci, leading to difficulties in distinguishing them from each other. A recent study has shown that some circRNAs can be specifically depleted by using base editing systems to target their predominantly back-splice sites for circularization

Minatory

污點(diǎn)

covert

教義

Book 2024Latest editionation, circRNA detection, sequence validation, quantification , techniques related to? gain- and loss-of-function approaches, circular RNA synthesis, split ligation, engineering, nanoparticle packaging, RNA modifications on circular RNA biogenesis, RNA translation potential, and vaccines based on ci

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1064-3745 ation advice from the experts.This second edition details new and updated methods on circular RNA. Chapter guide readers through circular RNA purification, .in silico. characterization, circRNA detection, sequence validation, quantification , techniques related to? gain- and loss-of-function approac

貪婪的人

candle

鴕鳥

Directed Circularization of a Short RNAelf-cleavage poorly. We designed an activator-polyamine complex to complete cleavage as a prerequisite for subsequent circularization. The developed protocol allows synthesizing circRNA without external enzymatic assistance and adds a controllable way of circularization to the existing methods.

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