Titlebook: Circadian Rhythms; Methods and Protocol Ezio Rosato Book 2007 Humana Press 2007 Arabidopsis thaliana.Microarray.PCR.Quantitative PCR.Vivo.W

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Analysis of Rhythmic Gene Expression in Adult , Using the Firefly Luciferase Reporter Genected during a specific time of the circadian day need to be sacrificed, followed by the extraction of clock gene products. This procedure makes it impossible to analyze molecular rhythms in an individual over time. To measure clock gene expression within the living animal, firefly luciferase can be

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Monitoring Circadian Rhythms in , Using Luciferase Reporter Genesnot display any obvious circadian rhythms; however, rhythms in gene expression can be readily detected. For this reason the promoters of rhythmically expressed genes have been fused to the firefly . gene. Using this reporter gene we have developed a number of automated techniques for monitoring luci

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Mutagenesis With , . pacemaker genes were discovered through the isolation of gene variants affecting the free-running period of the circadian pacemaker. There are different ways to mutagenize flies. An alkylating agent can be used to randomly alter the fly genome, or transposable elements can be mobilized to disrupt

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Microarrayshis technology, it is important to perform quality control experiments before engaging in genome-wide expression analysis. In this chapter, we provide an overview of quantitative polymerase chain reaction experiments using the ABI PRISM? 7900HT system for quality control of samples, and micorarray e

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