Titlebook: Circadian Clocks; Methods and Protocol Steven A. Brown Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Neurosp

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https://doi.org/10.1007/978-3-030-90398-5The forced swim and tail suspension tests are commonly used to determine the effects of circadian-related pharmacological, genetic, and environmental manipulations on depression-like behavior in rodents. Both tests involve scoring immobility of rodents in an inescapable condition. Here we describe how to set up and carry out these tests.

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Inductively Coupled Plasma Mass Spectrometry for Elemental Analysis in Circadian BiologyInductively coupled plasma mass spectrometry (ICP-MS) is a sensitive instrumental analysis technique used for multielemental and isotopic determination. Here we provide a sample preparation and circadian ICP-MS analysis protocol for use with mammalian tissues and cells, using mouse fibroblasts as a case study.

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Measuring the Effects of Circadian Rhythm-Related Manipulations on Depression-Like Behavior in RodenThe forced swim and tail suspension tests are commonly used to determine the effects of circadian-related pharmacological, genetic, and environmental manipulations on depression-like behavior in rodents. Both tests involve scoring immobility of rodents in an inescapable condition. Here we describe how to set up and carry out these tests.

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Circadian Clocks978-1-0716-0381-9Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-349-05789-4 randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.

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Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.

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The Assessment of Circadian Rhythms Within the Immune Systemainst pathogens) and the adaptive immunity (a more specific set of responses, which lead to immune memory). Here, to illustrate principles to be taken into account when working on circadian rhythms in immunology experiments, two protocols will be presented. The first one aims to analyze immune param

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Measuring Circadian Rhythms in Human Cellssources that bridge the gap between animal models and human subjects in basic as well as clinical research. The cells also retain molecular circadian clocks that reflect subject-specific differences in circadian physiology, and the cellular rhythms can be measured easily in large scale. This is a se