Titlebook: Circadian Clocks; Tsuyoshi Hirota,Megumi Hatori,Satchidananda Panda Book 2022 The Editor(s) (if applicable) and The Author(s), under exclu

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A Primer for Circadian Metabolic Profile Analysis Using Multi-platform Metabolomics,have been known to fluctuate in a circadian fashion for some time, recent studies have used metabolomics to expand the known cycling metabolome. Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are two primary analytical platforms readily utilized for metabolomics analyses. H

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Next-Generation Mice Genetics for Circadian Studies,lating the mammalian circadian system at the organism level. However, the time and space required to produce genetically modified mice depend heavily on the number of mice crosses required. Here we describe methods for producing gene knockout and knock-in mice without the need for crossing, namely,

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Mathematical Modeling of Circadian Rhythms, a research technique to both generate and test hypotheses. Mathematical model simulations have elucidated key processes controlling phase, amplitude, period, and synchrony in the circadian system from the molecular level to the organismal level. Through a series of three examples spanning a range o

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Trigeminal and Sphenopalatine Proceduresgm enables assessment of the effect of energy balance on the daily activity rhythms of an animal, including its decision to switch temporal niche. Adaptive behavioral strategies to cope with energetic challenges may vary depending on species, sex, age, and reproductive status. This chapter provides

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https://doi.org/10.1007/978-94-009-2321-8rinciples and guidelines for tissue sampling and data generation and analysis. This will ensure rigor in designing and performing experiments, ensure reproducibility, and also facilitate comparisons with existing and future datasets. This chapter outlines key principles and guidelines for experiment

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H. J. Graaf,C. J. M. Musters,W. J. Ter Keurss about valuable targets for shifting the clock. They have demonstrated how features of networks of oscillators lead to synchrony among the oscillators of the circadian system, and they have illustrated how different experimental protocols may result in seemingly contradictory conclusions about the

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