Titlebook: Chromosome Painting; Principles, Strategi Arun Kumar Sharma,Archana Sharma Book 2001 Springer Science+Business Media Dordrecht 2001 Chromos

反饋

纖細

欲望小妹

Concomitant

synovium

Chromosome painting in plants,hybridisation (GISH) with total genomic DNA to distinguish alien chromosomes on the basis of divergent dispersed repeats, ii) ‘Chromosomal . suppression’ (CISS) hybridisation with chromosome-derived DNA probes and blocking of interchromosomally dispersed repeats by total genomic or C..-1 DNA in exce

saturated-fat

Localisation of DNA sequences on plant chromosomes using PRINS and C-PRINS,d is suitable for most applications it is time-consuming and requires labelled probes. Recently, primed . labelling (PRINS) has been developed as an alternative to FISH. PRINS is based on annealing of unlabelled oligonucleotide primer(s) to chromosome DNA and its elongation by DNA polymerase in the

obsession

GISH technology in plant genome research,eloped in recent years. It has enabled plant chromosome and genome research to progress at phenomenal rate and its potential is far from exhausted. Genomic . hybridization has applications in characterization of genomes and chromosomes in hybrid polyploids, hybrid plants, partial allopolyploids, pol

Ballerina

CLAIM

乳汁

Chromosome painting as a tool for rice genetics and breeding,e applied even in the typically small chromosomes of rice. This manuscript describes in detail, highly reproducible, complete protocols for chromosome painting and GISH in rice chromosomes. Examples of useful applications of these methods are also presented.