Titlebook: Chromatin Protocols; Peter B. Becker Book 19991st edition Springer Science+Business Media New York 1999

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Nucleotide Excision Repair Coupled to Chromatin Assembly,on and repair. During nucleotide excision repair (NER), a major DNA repair pathway, rearrangements of the nucleosomal organisation are observed (.). These rearrangements can be envisioned as the rapid succession of disassembly and reassembly events. A tight co-ordination between the actual DNA repai

irradicable

Photolyase,e octamers on the DNA sequence (nucleosome positions) as well as structural and dynamic properties of nucleosomes may play important roles in gene regulation, replication and DNA repair. Conventional approaches to characterize chromatin structure include (partial) purification of chromatin and chara

BLANK

Transcriptional and Structural Analyses of Isolated SV40 Chromatin,, SV40 DNA is complexed with cellular histone and nonhistone proteins to form the episomal chromatin structure called a minichromosome (MC). MCs are therefore the viral template for both the host replication and host transcription machinery. The chromatin properties of MCs reflect transcriptionally

BET

Désordres sévères de l’avant-piedxperiments carried out in vitro, and they have important consequences for nucleosome function in vivo. This article will not discuss specific experimental methods; rather, it will summarize several aspects of the equilibrium and dynamic behavior of real nucleosomes that are of particular importance for studies of nucleosome structure and function.

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GLIB

aspersion

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切割

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Nucleosome Structure and Dynamics,ound to correspond to a reduction in the DNA linking number, Lk, of approx 26 (.), and the number of nucleosomes in the minichromosomes was approximately the same (.,.). Thus, each nucleosome reduces Lk by one (.., for Lk definition).