Titlebook: Chondrocytes; Methods and Protocol Tariq M. Haqqi,Véronique Lefebvre Book 2021 Springer Science+Business Media, LLC, part of Springer Natur

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MicroRNAs and Regulation of Autophagy in Chondrocytes,research of gene and miRNA expression in articular cartilage, in this chapter we provide a summary of the tools models to direct in vitro and in vivo studies aimed at gaining a better understanding of the regulatory roles of miRNAs in chondrocyte autophagy.

BILIO

Mitochondrial Biogenesis, Activity, and DNA Isolation in Chondrocytes,g pathways that are involved in disturbance of cartilage homeostasis. In this chapter, we describe the methods to evaluate mitochondrial biogenesis, activity and mitochondrial DNA (mtDNA) integrity in chondrocytes.

BABY

Isolation of Chondrocytes from Human Cartilage and Cultures in Monolayer and 3D, years, providing ways to obtain viable cells for tissue engineering and clinical applications. Here we describe a method to obtain populations of chondrocytes that are able to expand and maintain a native-like phenotype.

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MicroRNA In Situ Hybridization in Paraffin-Embedded Human Articular Cartilage and Mouse Knee Jointsdetection probe. Here, we provide the optimized miRNA ISH protocol for human cartilage and mouse whole knee joints that also entails the necessary steps for sample collection, processing, and preparation for high-quality ISH staining.

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1064-3745 expertsThis detailed book collects a variety of methods from investigators at the forefront of chondrocyte biology and pathology research that they have tailored for their research projects in order to tackle the unique properties and challenges of the chondrocyte and those of its tissue matrix. Di

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