Titlebook: Chlamydia trachomatis; Methods and Protocol Amanda Claire Brown Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 201

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Lecture Notes in Networks and Systemsare amenable for the routine genetic engineering of .. Here, we provide several detailed protocols for performing genetic analysis in . allowing investigators to elucidate how this obligate intracellular pathogen causes disease.

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Book 2019homatis.. These include techniques to identify?.Chlamydia trachomatis.?in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate?.Chlamydia trachomatis?.in molecular gene

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Antimicrobial Resistance Screening in , by Optimized McCoy Cell Culture System and Direct qPCR-Based. Here we describe how to approach antimicrobial susceptibility/resistance screening in . by using a McCoy cell culture system, optimized by a research group from Croatia, and direct qPCR-based monitoring of chlamydial growth, optimized by a research group from Hungary.

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Multilocus Sequence Typing (MLST) of ,ively constitute an allelic profile or sequence type (ST), a “molecular barcode” of the interrogated bacterial strain or a eukaryotic organism. Here, we describe the principles and molecular approaches for generating MLST data for an analysis of a bacteria in the order ., using a .-specific MLST scheme as an example.

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Forward and Reverse Genetic Analysis of ,are amenable for the routine genetic engineering of .. Here, we provide several detailed protocols for performing genetic analysis in . allowing investigators to elucidate how this obligate intracellular pathogen causes disease.