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Titlebook: Chimera Research; Methods and Protocol Insoo Hyun,Alejandro De Los Angeles Book 2019 Springer Science+Business Media, LLC, part of Springer

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發(fā)表于 2025-3-21 17:01:43 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Chimera Research
副標題Methods and Protocol
編輯Insoo Hyun,Alejandro De Los Angeles
視頻videohttp://file.papertrans.cn/226/225309/225309.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Chimera Research; Methods and Protocol Insoo Hyun,Alejandro De Los Angeles Book 2019 Springer Science+Business Media, LLC, part of Springer
描述.This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of na?ve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful .Methods in Molecular Biology. series format, chapters
出版日期Book 2019
關(guān)鍵詞Pluripotent Stem Cells; Somatic Cells; Whole-organ Transplantation; Xenobarrier; Parabiosis
版次1
doihttps://doi.org/10.1007/978-1-4939-9524-0
isbn_softcover978-1-4939-9526-4
isbn_ebook978-1-4939-9524-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Introduction to Health Physics,We provide methods for basic characterization of primate PS cell lines, which include immunostaining for pluripotency markers such as OCT4 and TRA-1-60, as well as in vivo teratoma formation assay. We provide methods for generating alternative PS cells including region-selective primed PS cells, two
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,Radioactive Atoms–Nature and Behavior,generate interspecies chimeras between humans and animals. While some jurisdictions prohibit the introduction of human PS cells into monkey preimplantation embryos, other jurisdictions may permit and even encourage such experiments. Therefore, it is useful to consider blastocyst complementation expe
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Book 2019the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful .Methods in Molecular Biology. series format, chapters
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Frontiers of Pluripotencyhas been defined by competence to form germline chimeras and governance by unique KLF-based transcription factor (TF) circuitry, whereas primed state is distinguished by an inability to generate chimeras and alternative TF regulation. However, the discovery of many alternative PS cell states challen
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Embryonic Chimeras with Human Pluripotent Stem CellsWe provide methods for basic characterization of primate PS cell lines, which include immunostaining for pluripotency markers such as OCT4 and TRA-1-60, as well as in vivo teratoma formation assay. We provide methods for generating alternative PS cells including region-selective primed PS cells, two
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