Titlebook: Chemokine Protocols; Amanda E. I. Proudfoot,Timothy N. C. Wells,Christi Book 2000 Humana Press 2000

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書目名稱Chemokine Protocols影響因子(影響力)




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書目名稱Chemokine Protocols網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




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Yourself

Chemokine Expression in Insect Cells,ay be predicted from deduced amino acid sequence, a rigorous proof of identity of a gene will require a demonstration of the appropriate biological activities of gene products. Thus, efficient expression of recombinant proteins of cloned genes is essential to obtain proteins sufficient for detailed

周興旺

草率男

Expression of Chemokines in the Periplasmic Space of E. coli,ations, in the case of RANTES, and with a yield of several hundred micrograms from a 1-L culture. Other expression systems that are generally used for the production of chemokines have several disadvantages. Bacterial expression in the cytoplasm of . (.,.) gives large yields, but sometimes leads to

Atrium

Synthesis of Chemokines,ning has resulted in an explosion in the number of new chemokines from 1995-1998. However, for studies of the protein, knowing the DNA sequence is only the first step. The chemokine must be generated and in its correctly processed and folded form, and then purified to homogeneity. Expression of the

Atrium

Identification of Novel Chemokines From Expressed Sequence Tag Databases,hree-dimensional subunit structure. The original members of the superfamily, Interleukin-8 and MCP-1 were purified over 10 years ago. Following on from these discoveries, many more of these proteins were identified because of their ability to selectively recruit and activate specific leukocyte popul

EWER

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有法律效應(yīng)

Generation of Stable Cell Lines Expressing Chemokine Receptors, expressing specific chemokine receptors greatly facilitates chemokine receptor characterization, particularly in terms of ligand binding specificity, analysis of signal transduction pathways, and for diverse functional assays ranging from chemotaxis to HIV infection. The availability of stable cell