Titlebook: Chaperones; Methods and Protocol Stuart K. Calderwood,Thomas L. Prince Book 2023Latest edition The Editor(s) (if applicable) and The Author

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Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Networtwork of chaperone interactions, known as the chaperome, contributes significantly to the proteotoxic cell response and the malignant phenotype in cancer. We identified a potent microRNA, miR-570 that could bind the 3′untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis. Here, we wil

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Complementation Assays for Co-chaperone Function,s for testing the co-chaperone function of JDPs. In these assays, an exogenously expressed novel JDP is tested for its ability to functionally substitute for a non-expressed or nonfunctional endogenous JDP(s) by reversing a stress phenotype. For example, the in vivo functionality of prokaryotic JDPs

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Detecting Posttranslational Modifications of Hsp90 Isoforms,ep carcinogenesis. There are multiple Hsp90 isoforms including the cytosolic Hsp90α and Hsp90β as well as GRP94 located in the endoplasmic reticulum and TRAP1 in the mitochondria. The chaperone function of Hsp90 is linked to its ability to bind and hydrolyze ATP. Co-chaperones and posttranslational