Titlebook: Chaperones; Methods and Protocol Stuart K. Calderwood,Thomas L. Prince Book 2023Latest edition The Editor(s) (if applicable) and The Author

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1064-3745 ation advice from the expertsThis second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response;

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Natural Numbers Just Aren’t Enoughme study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe a protocol for detection and composition analysis of epichaperomes in cell homogenates through native polyacrylamide gel electrophoresis.

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Transfection and Thermotolerance Methods for Analysis of miR-570 Targeting the HSP Chaperone Networcer. We identified a potent microRNA, miR-570 that could bind the 3′untranslated regions of multiple HSP mRNAs and inhibit HSP synthesis. Here, we will introduce the transfection and thermotolerance methods for analysis of miR-570 targeting the HSP chaperone network.

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