Titlebook: Cervical Cancer; Methods and Protocol Daniel Keppler,Athena W. Lin Book 2015 Springer Science+Business Media New York 2015 Cervix.CxCA.Drug

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Retroviral Expression of Human Cystatin Genes in HeLa Cellsucts that have negative effect on cell growth and viability. Here, we describe the retroviral transfer of cystatin cDNAs using HEK293-derived Phoenix packaging cells and human HeLa cervical carcinoma cells as target cells.

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A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for thet way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler. 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.

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