Titlebook: Cellular and Molecular Methods in Neuroscience Research; Adalberto Merighi,Giorgio Carmignoto Book 2002 Springer Science+Business Media Ne

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Tract Tracing Methods at the Ultrastructural Level,nervous system. Tract tracing methods are among the best approaches to elucidate the connections between neurons located in distinct areas of the nervous system since they allow an easy recognition of large neuronal populations and their structural characterization. Although the bulk of information

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In Vivo Analysis of Cell Proliferation and Apoptosis in the CNS,tion of the various tissues and organs that form the animal body. Apoptosis is a gene-regulated process of programmed cell death (PCD) that plays fundamental roles in several normal and pathological conditions (56,126). This form of “cell suicide” is most often detected during embryonic development,

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Confocal Imaging of Calcium Signaling in Cells from Acute Brain Slices,nges in [Ca.]. in individual cells. Techniques for performing such experiments are well documented (5,9,14,15). More recent advancements in confocal laser scanning microscopy (CLSM) have made it possible to perform these measurements even within tissue slices. Preparation of the CLSM tissue and perf

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