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Titlebook: Cells and Culture; Proceedings of the 2 Thomas Noll Conference proceedings 2010 Springer Science+Business Media B.V. 2010 Proteomics.bioinf

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發(fā)表于 2025-3-21 16:06:31 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Cells and Culture
副標(biāo)題Proceedings of the 2
編輯Thomas Noll
視頻videohttp://file.papertrans.cn/223/222961/222961.mp4
概述A Comprehensive review of the latest research and developments in the field of animal cell technology..A unique collection of papers on animal cell technology for industrialists..Practical information
叢書名稱ESACT Proceedings
圖書封面Titlebook: Cells and Culture; Proceedings of the 2 Thomas Noll Conference proceedings 2010 Springer Science+Business Media B.V. 2010 Proteomics.bioinf
描述Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cel
出版日期Conference proceedings 2010
關(guān)鍵詞Proteomics; bioinformatics; biotechnology; cell; cell culture; glycoprotein; microarray; physiology; protein
版次1
doihttps://doi.org/10.1007/978-90-481-3419-9
isbn_softcover978-94-024-0520-0
isbn_ebook978-90-481-3419-9
copyrightSpringer Science+Business Media B.V. 2010
The information of publication is updating

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Targeted Gene Knockdown Effects on Recombinant Protein Secretion in CHO Cellss of the LEAP instrument to quantify recombinant protein secretion from individual cells. The same siRNAs were co-transfected into parental CHO cells along with the IgG light and heavy chain genes to confirm the results using a transient expression system. These experiments further our understanding
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Towards the Use of CHO Produced Recombinant Extracellular Matrix Proteins as Bioactive Elements in ample fluorescence measurement. Subsequently, we cloned genes encoding two main ECM proteins (collagen I and tropoelastin) into mammalian expression vectors. We showed that recombinant elastin and collagen are both expressed and secreted by CHO DG44 cells. In a next step, CHO cells genetically modifi
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Cell Xpress? Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using exhibited different degrees of secretion heterogeneity. In conclusion, Cell Xpress? enables high-throughput multi-parameter clone analysis and selection based on IgG secretion intensity and heterogeneity. Single-cell clones may develop secretion heterogeneity during expansion, which may contribute t
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Gene Modified Hematopoietic Stem Cells for the Treatment of Primary Immunodeficiency Diseasese protein products of PID genes are highly expressed in the hematopoietic system, addition of correct copies of the gene to hematopoietic stem cells (HSC) can be expected to restore immune function. Following recent advances in the technology of gene transfer three PID, X-linked severe combined immu
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https://doi.org/10.1007/978-3-319-73392-0s of the LEAP instrument to quantify recombinant protein secretion from individual cells. The same siRNAs were co-transfected into parental CHO cells along with the IgG light and heavy chain genes to confirm the results using a transient expression system. These experiments further our understanding
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