Titlebook: Cell Migration in Three Dimensions; Coert Margadant Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids single cells on two-dimensional surfaces, as well as in multicellular three-dimensional (3D) structures and in tissue in vivo. 3D multicellular cultures can reveal how the topology of the extracellular matrix and cell-cell contacts influence subcellular mRNA distributions. Here we describe a method

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Book 2023igration in Three Dimensions. provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes.?.Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.

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1064-3745 processes.?.Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.978-1-0716-2889-8978-1-0716-2887-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Probing the ER-Focal Adhesion Link During Cell Migrationion. Our analysis pipeline includes two examples showing physiological conditions and disruption of ER-FA contacts upon nocodazole treatment. The described method can be adapted to different cell lines.

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