Titlebook: Cell Fusion; Overviews and Method Kurt Pfannkuche Book 2015Latest edition Springer Science+Business Media New York 2015 cell fuse.cell orga

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https://doi.org/10.1007/978-981-4451-94-9ly slightly attached to the dish surface maintaining the spherical shape. Observed high fusion yields up to 50 % obtained by MAM in situ by dual-color fluorescence microscopy are among the highest in field of electrofusion. The method can be readily adapted to other anchorage-dependent cell lines.

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Detection of Fusion Events in Mammalian Skeletal Muscle, levels of genes involved and associated with cell fusions events. In addition to the investigation if cell fusion markers in skeletal muscle tissue, in vitro cell culture systems (e.g., C2C12 cells) can be used to study cell fusions events in a highly standardized system in order to obtain detailed

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Analyzing Cell Fusion Events Within the Central Nervous System Using Bone Marrow Chimerism,n fluorescent protein expressing sex-mismatched bone marrow. These chimeric mice are known to represent an excellent model for studying bone marrow cell migration and infiltration throughout the body, while in parallel, as will be described here, also provide a means to neatly analyze both bone marr

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,Mouse Embryos’ Fusion for the Tetraploid Complementation Assay,. In addition chimeras provide a powerful tool to study the cell lineage and to analyze complex phenotypes of mutant mice. Mouse chimeras with tetraploid embryos are used to rescue extraembryonic defects, to analyze an impact of gene function on specific lineage, to study the interaction between emb

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Generation of Mouse Chimeras with High Contribution of Tetraploid Embryonic Stem Cells and Embryonills with pre-implantation embryos to generate chimeras, thus facilitating the creation of a bridge between in vitro and in vivo investigations. Genomic manipulation using ES cells and homologous recombination is one of the most outstanding scientific achievements, resulting in the generation of anim