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Titlebook: Cell Cycle Synchronization; Methods and Protocol Gaspar Banfalvi Book 2017Latest edition Springer Science+Business Media New York 2017 Unic

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發(fā)表于 2025-3-21 16:14:49 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Cell Cycle Synchronization
副標(biāo)題Methods and Protocol
編輯Gaspar Banfalvi
視頻videohttp://file.papertrans.cn/223/222795/222795.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Cell Cycle Synchronization; Methods and Protocol Gaspar Banfalvi Book 2017Latest edition Springer Science+Business Media New York 2017 Unic
描述This second edition volume provides detailed protocols on the theoretical background of cell cycle synchronization procedures and instructions on how to implement these techniques. The chapters in?.Cell Cycle Synchronization: Methods and Protocols, Second Edition.?cover subjects such as: physical fractionations including centrifugal elutriation of healthy and apoptotic cells, and nuclei of mammalian cells; large scale mitotic cell synchronization; chromosome formation during fertilization in eggs; synchronization of unicellular organisms; hematopoietic stem cells used to improve the engraftment in transplantation; and cell cycle control. Written in the highly successful?.Methods in Molecular Biology?.series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Practical and comprehensive,?.Cell Cycle Synchronization: Methods and Protocols, Second Edition.?is a valuable resource for PhD students and postdoctoral fellows, and researchers interested in general science, pharmacy, medicine and public health, compute
出版日期Book 2017Latest edition
關(guān)鍵詞Unicellular organisms; Transfected cells; Stem cells; Physical fractionation; centrifugal elutriation of
版次2
doihttps://doi.org/10.1007/978-1-4939-6603-5
isbn_softcover978-1-4939-8248-6
isbn_ebook978-1-4939-6603-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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發(fā)表于 2025-3-21 21:29:57 | 只看該作者
Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriationparticles, with minimal perturbation of cellular functions. The physical characteristics of cell size and sedimentation velocity are operative in the technique of centrifugal elutriation also known as counterstreaming centrifugation. The elutriator is an advanced device for increasing the sedimentat
板凳
發(fā)表于 2025-3-22 02:35:17 | 只看該作者
Image Cytofluorometry for the Quantification of Ploidy and Endoplasmic Reticulum Stress in Cancer Ceed by asymmetric divisions and chromosome loss. Increases in ploidy are consistently accompanied by the activation of an endoplasmic reticulum (ER) stress response, resulting in the translocation of calreticulin to the outer surface of the plasma membrane where it stimulates anticancer immune respon
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發(fā)表于 2025-3-22 08:20:57 | 只看該作者
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發(fā)表于 2025-3-22 13:32:27 | 只看該作者
Synchronization of Mammalian Cell Cultures by Serum Deprivationhe cycle. Procedures to arrest cultured cells in specific phases of the cell cycle may be termed in vitro synchronization. The procedure described here was developed for the study of primary astrocytes and a glioma cell line, but is broadly applicable to other mammalian cells. Its application allows
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發(fā)表于 2025-3-22 19:22:38 | 只看該作者
DNA Damage Response Resulting from Replication Stress Induced by Synchronization of Cells by Inhibithidicolin or excess of thymidine the cells that become arrested at the entrance to S-phase upon release from the block initiate progression through S then G. and M. However, exposure to these inhibitors at concentrations commonly used to synchronize cells leads to activation of ATR and ATM protein k
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發(fā)表于 2025-3-23 01:06:10 | 只看該作者
Chromosome Formation During Fertilization in Eggs of the Teleost , been accumulated by various experiments using inhibitors to affect formation and behavior of chromosomes in the cycle of cell proliferation. Based on experimental results on meiosis and early stages of development of the teleost ., we discuss the roles of the activities of histone H1 kinase, microt
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發(fā)表于 2025-3-23 01:43:31 | 只看該作者
Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyraten MDBK cells. We explore the possibility of using butyrate-blocked cells to obtain synchronized cells and we characterize the properties of butyrate-induced cell cycle arrest. The site of growth inhibition and cell cycle arrest was analyzed using 5-bromo-2′-deoxyuridine (BrdU) incorporation and flow
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發(fā)表于 2025-3-23 06:28:33 | 只看該作者
Chemically Induced Cell Cycle Arrest in Perfusion Cell Culturehat would meet all requirements does not exist, we have established a long-term scanning-perfusion platform that: (a) replaces old medium with fresh one, (b) bypasses physical contact with the cell culture during continuous cell growth, (c) provides uninterrupted photomicrography of single cells, an
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