Titlebook: Cell Cycle Checkpoints; Methods and Protocol James J. Manfredi Book 2021 The Editor(s) (if applicable) and The Author(s) 2021 Flow cytometr

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Book 2021-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Cell Cycle Checkpoints: Methods and Protocols. is a valuable resource for researcher interested in learning more about this developing field..

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Open Multithreaded Transactionso DNA damage. Cell-free extracts derived from the eggs of . remain a robust and versatile system to decipher the biochemical steps underlying this essential characteristic of all cells, critical for genome stability.

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Modeling Aerosol Drug Delivery,y characterized by the exposure of the endoplasmic reticulum protein calreticulin on the cell surface. Here we describe an immunofluorescence-based cytofluorometric technique to detect calreticulin exposure on tumor cells exposed to drugs that induce mitotic catastrophe.

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DNA Affinity Purification: A Pulldown Assay for Identifying and Analyzing Proteins Binding to Nuclerves as a link for indirect transcriptional repression of target genes by the tumor suppressor p53. In general, the methods described can be applied for the identification and analysis of proteins binding to DNA.

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Detection of Post-translationally Modified p53 by Western Blotting, p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.

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