Titlebook: Cell Culture Technology; Cornelia Kasper,Verena Charwat,Antonina Lavrentiev Textbook 2018 Springer International Publishing AG, part of Sp

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Cell Culture Media,nts for individual cell types from different tissues will be covered with a focus on stem and progenitor cells. Moreover, approaches toward the derivation of defined media and the supplementation of serum additives are described. The relevance of physical parameters such as osmolarity and buffer sys

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,Establishment of Tumor Cell Lines: From Primary Tumor Cells to a?Tumor Cell Line,sible in order to obtain optimal research conditions. Cell lines are defined as continuously growing cell cultures, with unlimited proliferation. During cell line establishment scientists need to be cognizant of numerous contaminants including bacterial, viral and cell-cell interaction along with th

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The Third Dimension in Cell Culture: From 2D to 3D Culture Formats,rast, the cultivation of cells in a three-dimensional (3D) environment can help to generate a physiologic environment. This chapter describes the fundamental biological and technological differences of 2D to 3D cultivation and gives an overview on important 3D cell culture techniques. Different appr