Titlebook: Cell Culture Techniques; Michael Aschner,Lucio Costa Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation,developmental processes, such as NPC proliferation, migration, neuronal- and oligodendrocyte differentiation, as well as thyroid hormone (TH)-dependent oligodendrocyte maturation. By including viability and cytotoxicity assays into the workflow, the assays allow the distinction of specific DNT from

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Bereavement

Rat Brain Slices: An Optimum Biological Preparation for Acute Neurotoxicological Studies,in living animals. Here we describe several advantages of this preparation for simple acute neurotoxicological studies and some of the experimental approaches that, in our experience, can reveal, in a rapid manner, neurotoxic and neuroprotective profiles of several molecules in different acute toxic

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miRNA as a Marker for In Vitro Neurotoxicity Testing and Related Neurological Disorders,n) injury/toxicity. In this chapter, the importance of miRNA for neural development, neurotoxicity, and neurodegeneration is discussed; the critical steps of miRNA profiling in tissues/cells as well as in biofluids are described; the challenges and options of bioinformatic data analysis are delibera

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Sigmoidoscopy

S. A. Zenios,G. M. Chertow,L. M. Weintion of permeability coefficients (.) for small molecules, and assessment of efflux transporter activity. We describe these methods in the context of BBB endothelial cells derived from human-induced pluripotent stem cells (iPSCs), a model commonly employed in our research group.

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